Increased Plasminogen Activator Inhibitor-1 May Explain Dexamethasone-induced Thrombosis as Site of Intraluminal Wire Injury
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چکیده
specimens and using immunomagnetic beads (Miltenyi). Commercially available aortic medial SMC from six healthy donors were purchased from Promocell. Cells were used consistently at passage 3. We used two-dimensional electrophoresis with digital image analysis (SameSpots, Non-linear Dynamics) and tandem mass spectrometry to detect changes in proteome of atherosclerotic SMC. Results Analysis of 2D gel images revealed 29 proteins with a statistically significant difference in expression between medial and plaque SMC (p<0.05). Plaque SMC had decreased expression of mitochondrial protein ATP synthase subunit b but an increase in the oxidised form of peroxiredoxin-4, suggesting decreased mitochondrial function, possibly owing to oxidative stress. Glycolytic enzyme pyruvate kinase was also increased by 50% in plaque SMC. Furthermore, differences in protein expression between SMC from symptomatic and asymptomatic patients were also found. Plaque SMC from symptomatic patients exhibited increased expression of heat shock protein-60 and decreased levels of the antiinflammatory protein annexin I (p<0.05), compatible with a proinflammatory behaviour. These findings were confirmed by immunoblotting. Conclusions Our data demonstrate that plaque-derived SMC are exposed to higher levels of oxidative stress than control SMC. Differences between SMC from symptomatic and asymptomatic patients appear to reflect proinflammatory changes associated with plaque instability.
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تاریخ انتشار 2010